ponceau stain purpose

These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. 18 0 obj Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors. The cookies is used to store the user consent for the cookies in the category "Necessary". Prepare working solutions immediately before use. Privacy Policy | Terms of Use | Site Map, High-quality and affordable reagents, imagers, and qPCR systems, At this time our store is only available to customers within the United States. Lbij>Owq4S!;y^RFAv:A'x* Q#8$B1Tp}q)=-?Qf2_Fw"M=yAC^'9}ya&uYtML[&'as )b8K4 IrEV_}k-dc)QkQRh >*Oz.ZwmyYN[!#.L2_C}0~! endobj /ExtGState <>>>/Group <> 3 0 obj endobj To measure transferred protein quantitatively in fluorescent western blotting, we recommend the VersaBlot Total Protein Normalization Kits. In a number of Alexa Fluor SE reactive dyes, the SE group is derived from an aromatic carboxylic acid, while in all of Biotiums CF dyes the SE group is prepared from an aliphatic carboxylic acid. Concentrated coelenterazine stock solutions (typically 1-100 mg/mL) should be prepared in ethanol or methanol; do not use DMSO or DMF to dissolve coelenterazines, because these solvents will oxidize the compounds. The stain binds to proteins on the membrane reversibly and works well for nitrocellulose and PVDF membranes. Ensure the quality of protein transfer from gel to membrane before proceeding with your Western blot. A visible, blue protein gel staining solution. 12 0 obj Ponceau S is a classic visible red protein dye. My product arrived at room temperature or thawed, but its supposed to be stored at 4C or -20C. If your institution requires you to document expiration date based on date of manufacture for reagents, please contact techsupport@biotium.com for assistance. 16 0 obj endobj <> This is a rapid and reversible staining used for locating protein bands on Western blots and comparing protein band intensity. endobj AM esters and diacetate compounds should be dissolved in a solution that is free of serum, because serum could contain esterases that would hydrolyze the compound. Esters are not stable in aqueous solution. Ethanol or methanol stocks of coelenterazine can be stored at -20C or below for six months or longer; alcohol stocks may evaporate during storage, so use tightly sealing screw cap vials and wrap the vials with Parafilm for long term storage. Most of our products are stable at room temperature for many days, but we recommend storage at 4C or -20C to prolong shelf life. <> endstream A note on CF dye succinimidyl ester stability x3R235W(*T0PR0T(Y@@QC= P AJ 1RpW Like the succinimidyl ester dyes, these dyes are also susceptible to hydrolysis, although generally to a much lower degree. endobj endobj The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". 8 0 obj endstream Some kits have an expiration date printed on the kit box label, this is the guaranteed shelf life date calculated from the day that the product shipped from our facility. I purchased coelenterazine, but it has red/brown flecks. By clicking Accept, you consent to the use of ALL the cookies. For example, the percent of active Alexa Fluor 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the manufacturers product datasheet). The staining is reversible and membranes can be used immediately for Western blotting after de-staining. Other reactive dyes endobj Ponceau S staining is fast, easy and does not interfere with downstream western blotting. <>/Font <>/ProcSet [/PDF /Text ] 14 0 obj The guaranteed shelf life from date of receipt for bioscience kits is listed on the product information sheet. stream Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit (50 Preps), Minute Detergent-Free Single Nuclei Isolation Kit (20 Preps), Minute Total Protein Extraction Kit for Animal Cultured Cells and Tissues (50 Preps), Minute Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps), Minute Lysosome Isolation Kit for Mammalian Cells/Tissues (20 Preps), Minute Detergent-Free Protein Extraction Kit for Animal Tissues and Cultured Cells(50 Preps), Minute Total Protein Extraction Kit for Plant Tissues (50 Preps), Minute Detergent-Free Plant Protein Extraction Kit (50 Preps), Minute Total Protein Extraction Kit for Mass Spectrometry (50 Preps), Minute Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes (20 Preps, Minute Bacterial Total Protein Extraction Kit (50 Preps), Minute Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps), Minute Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps), Protein Extraction Kit for Hair and Nails (20 Preps), Minute Protein Extraction Kit for Fixed and Embedded Tissues (20 Preps), Minute Total Protein Extraction Kit for Skin Tissue (50 Preps), Minute Total Protein Extraction Kit for Bone Tissue (50 Preps), Minute Total Protein Extraction Kit for Blood Vessels (50 Preps), Minute Total Protein Extraction Kit for Tendons (50 Preps), Minute Total Protein Extraction Kit for Intestines and Mesentery (50 Preps), Minute Total Protein Extraction Kit for Muscles (50 Preps), Minute Total Protein Exatraction Kit for Insects (50 Preps), Minute Histone/DNA Binding Protein Extraction Kit (50 Preps), Minute Protein/Nucleic Acid Extraction Kit from Gels (20 Preps), Minute Adipose Tissue Fractionation Kit (20 Preps), Minute Chloroplast Isolation Kit (50 Preps), Minute Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps), Minute Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps), Minute Cytosolic Proteasome Enrichment Kit (20 preps), Minute Detergent-Free Nuclear Matrix Protein Extraction Kit (For Mammalian Cultured Cells/Tissues) (20 Preps, Minute Endosome Isolation and Cell Fractionation Kit (20 Preps), Minute Golgi Apparatus Enrichment Kit (20 Preps), Minute High Fidelity ER Isolation Kit for Liver Tissue (20 Preps), Minute High-Efficiency Exosome Precipitation Reagent (20 ml), Minute High-Efficiency Saliva Exosome Isolation Kit (50 Preps), Minute Mitochondria Isolation Kit for Muscle Tissues/Cultured Muscle Cells, Minute Mitochondria Isolation Kit for Mammalian Cells and Tissues (50 Preps), Minute Nuclear Proteasome Enrichment Kit (20 preps), Minute Nuclear Envelope Protein Extraction Kit (50 Preps), Minute Plant Cytosolic and Nuclear Protein Isolation Kit (Need 150 mg Raw Material, 20 preps), Minute Plant Endosome Enrichment Kit (20 preps), Minute Plant ER Enrichment Kit (20 preps), Minute Plant Golgi Apparatus Enrichment Kit (20 preps), Minute Plant Lipid Raft Isolation Kit (20 preps), Minute Plant Microsomal Membrane Extraction Kit (50 Preps), Minute Plant Plasma Membrane Protein Isolation Kit (50 Preps), Minute Plasma Membrane-Derived Lipid Raft Isolation Kit (20 preps), Minute Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues, Minute Yeast Mitochondria Enrichment Kit (50 Preps), Minute Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps), Minute Single Cell Isolation Kit for Fresh and Fixed Tissues (50 Preps), Minute Detergent-Free Nuclei Isolation Kit (20 Preps), Minute Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes (20 preps), Minute Single Nucleus Isolation Kit for Neuronal Tissues/Cells (20 preps), Minute Single Nucleus Isolation Kit for Tissues/Cells, Minute Native Protein Precipitation Kit from Saliva, Minute Organic Solvent-Free Milk Lipid Depletion Kit, Minute Albumin Depletion Reagent for Plasma and Serum (20 ml), Minute Denaturing Protein Solubilization Reagent (10 ml), Minute High-Efficiency Protein Precipitation Kit (30 ml), Minute Non-Denatured Protein Solubilization Reagent (10 ml), Minute Protein Solubilization Reagent for MS (2 ml), Minute Western Blot Stripping Solution (250 ml). stream Prepare concentrated D-luciferin stock solutions (typically 1-100 mg/mL) in water, and store in aliquots at -20C or below for six months or longer. <> Prepare working solutions in aqueous buffers immediately before use. Certificate No. 90082). Propylene glycol also can be used as a solvent to minimize evaporation. These cookies ensure basic functionalities and security features of the website, anonymously. Expiration date based on date of manufacture (DOM) endobj It does not store any personal data. Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. Succinimidyl esters are generally susceptible to hydrolysis, which can result in lower labeling efficiency. Antibodies and other conjugates Biotium guarantees the stability of chemicals, dyes, and gel stains for at least a year from the date you receive the product. 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They are stable for up to five hours in aqueous solution. stream It is not recommended for positively charged membranes such as nylon membranes. Succinimidyl esters (SE) should be dissolved in a solution that is free of amine-containing compounds like Tris, glycine, or protein, which will react with the SE functional group. Analytical cookies are used to understand how visitors interact with the website. Be the first to review Azure Ponceau Stain, Western Blotting Buffers, Reagents, and Accessories, Azure Biosystems Western Blotting Workflow. It produces reddish-pink-stained bands on membranes, which is useful because it does not have a deleterious effect on the property of proteins. This does not affect product stability or performance. endobj Products with recommended storage at ultra low temperature (-70C) that also ship on dry ice should arrive frozen. You also have the option to opt-out of these cookies. endobj Heavily sulfonated dyes, such as the Alexa Fluor dyes, DyLight dyes and IRDyes are particularly hygroscopic, worsening the hydrolysis problem. endstream This cookie is set by GDPR Cookie Consent plugin. D-luciferin is stable in solid form and as a concentrated stock solution when stored as recommended; it is not stable at dilute working concentrations in aqueous solution. You can quickly and easily check that protein transfer was even across the entire blot, with no bubbles or other transfer artifacts present. Kits often are functional for significantly longer than the guaranteed shelf life. 13 0 obj See our Western Blotting Technology page to learn about more products for optimal western blots. Manual & Protocol |Material Safety Data Sheets (MSDS). Azure Ponceau is a reversible stain that detects total protein on both nitrocellulose and PVDF membranes. Therefore, to save on shipping costs, products with recommended storage at 4C or -20C may ship at ambient temperature or with an ice pack. This does not affect product stability or performance. This cookie is set by GDPR Cookie Consent plugin. 15 0 obj x3R235W(*T0PR0T(Y@@QC= P AJ 1QpW The cookie is used to store the user consent for the cookies in the category "Analytics". For lyophilized antibodies, we recommend reconstituting the antibody with glycerol and antimicrobial preservative like sodium azide for the longest shelf life (note that sodium azide is not compatible with HRP-conjugates). <> stream Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. They are stable in solid form when stored as recommended. Where can I find the expiration date or shelf life of a product? Fluorescent compounds should be protected from light for long term storage. <> x3R235W(*T0PR0T(Y@@QC= P AJ 1UpW endobj <> See the product information sheets for specific reactive dyes more information. The cookie is used to store the user consent for the cookies in the category "Other. ; fI+j2]Q*oNAVi#F*kNx?[W"Win]tcPqQ2/bINwm"b"]\?X[t}azsAYL@X"6*4(*R/ \HQ0 gu4PzwtQbCn+Z}hA|U0N:nLjuUWwl 7oe}DD:3fU- ^d~y/DtgAo|V1a8`2#(Nr5z <> You can also download our Product Storage Statement here. The cookie is used to store the user consent for the cookies in the category "Performance". Fast protein staining in a single step without fixation or washing. Antibodies and other conjugates often are functional for significantly longer than the guaranteed shelf life. x3R235W(*T0PR0T(Y@@QC= P AJ 1WpW Diacetate-modified dyes, like ViaFluor 405, CFDA, and CFDA-SE cell viability/cell proliferation dyes. Succinimidyl esters (SE, also known as NHS esters), such as our amine-reactive dyes Necessary cookies are absolutely essential for the website to function properly. If a product you received was shipped on dry ice and thawed during transit, please contact customer service at order@biotium.com. Ester dyes are stable in solid form as long as they are protected from light and moisture. /ExtGState <>>>/Group <> If you have an older kit in storage that you wish to use, we recommend performing a small scale positive control experiment to confirm that the kit still works for your application before processing a large number of samples or precious samples. <> Esters should be diluted in aqueous solution immediately before use. stream If you have a Biotium compound that has been in storage for longer than one year that you wish to use, we recommend performing a small scale positive control experiment to confirm that the compound still works for your application before processing a large number of samples or precious samples. Bioscience kits 1 0 obj x3R235W(*T0PR0T(Y@@QC= P AJ 1SpW x3R235W(*T0PR0T(Y@@QC= P AJ 1TpW If a product you received was shipped on dry, ice and thawed during transit, please contact customer service at, Membrane & Cell Surface Stains Comparison, EvaEZ Fluorometric Polymerase Activity Assay, View all in Nucleic Acid Gel Stains & Accessories, View all in Nucleic Acid Quantitation & Extraction, Overview of Antibody & Protein Labeling Kits, Mix-n-Stain Small Molecule Labeling Kits, View all in Antibody & Protein Labeling Kits, Dyes For Labeling Carbonyls & Carbohydrates, Tyramides & Tyramide Signal Amplification Kits, View all in Reactive CF Dyes, Other Reactive Dyes & Biotinylation Reagents.

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